Figure 5.

Exogenous expression of DRP1C rescues drp1A-2 seedling lethality. A, Schematic of the four constructs used for complementation analysis. B, Immunoblot of total protein extracts from drp1A-2 (lanes 1–24) or wild-type (lanes 25 and 26) seedlings expressing ApA-myc (lanes 1–9), ApC-myc (lanes 10–16), 35pA-GFP (lanes 17–23, 26), or 35pC-GFP (lane 24). The top blot was blotted with anti-DRP1A specific antibodies, the middle blot with anti-myc (left) or anti-GFP (right) antibodies, and the bottom blot with anti-PUX1 antibodies (loading control). All lines express the transgene approximately equally well. The bands in the anti-DRP1A blot were DRP1A-GFP (top), DRP1A-myc (middle), and native, untagged DRP1A (bottom). A cross-reactive band is indicated by <. C, Histogram indicating the percentage of seedlings of individual lines (with the genotype indicated) that survived and developed a second set of true leaves on one-half-strength Murashige and Skoog, 0.6% agar without Suc.