Table II.
Analysis of the genetic transmission of VPS34/vps34-1 and VPS34/vps34-2
The inheritance of vps34 was analyzed by genotyping F1 progeny of the denoted crosses. The TE was calculated according to Howden et al. (1998): TE (%) = (observed PCR+/observed PCR−) × 100. Col0, Ecotype Columbia wild type.
Genotype (Female × Male) | PCR+ | PCR− | TE (%) |
---|---|---|---|
VPS34/vps34-1 × Col0 | 27 | 26 | 103.9 |
Col0 × VPS34/vps34-1 | 0 | 48 | 0.0 |
VPS34/vps34-1 × gl/gl | 52 | 52 | 100.0 |
gl/gl × VPS34/vps34-1 | 2 | 128 | 1.6 |
VPS34/vps34-2 × gl/gl | 90 | 74 | 121.6 |
gl/gl × VPS34/vps34-2 | 0 | 106 | 0.0 |