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. 2008 Aug;147(4):2179–2193. doi: 10.1104/pp.108.118570

Figure 3.

Figure 3.

Up-regulation of c3 at low temperature involves its promoter/5′-UTR. A, The chimeric construct Hsp70A-RbcS2pro:Ble-cRluc:C3-3′-UTR of pSK27 (see “Materials and Methods”) that was used for transformation resulting in transgenic lines crluc1 and crluc2 is shown schematically. ORF, Open reading frame. B, Measurements of cRLUC activities in cells (crluc1 and crluc2) grown at low and high temperature. Transgenic lines crluc1 and crluc2 that express crluc along with the 3′-UTR of c3 were grown at either 18°C or 28°C and used at LD2 to measure cRLUC activities (n = 3) according to Kiaulehn et al. (2007). C, The chimeric construct C3pro-5′-UTR:Ble-cRluc:C3-3′-UTR of pSK36 (see “Materials and Methods”) that was used for transformation resulting in transgenic lines crluc3 and crluc4 is schematically shown. D, Measurements of cRLUC activities in cells (crluc3 and crluc4) grown at low and high temperature. Transgenic lines crluc3 and crluc4 that express crluc under the control of the c3 promoter/5′-UTR along with the 3′-UTR of c3 were grown at either 18°C or 28°C and used at LD2 to measure cRLUC activity according to Kiaulehn et al. (2007).