Figure 6.

Up-regulation of c3 involves predominantly an E-box. A, Based on the shortened c3 promoter region, the E-box was replaced by six nucleotides representing a HindIII restriction site (pD26; see “Materials and Methods”). B, Measurements of cRLUC activities in cells (crluc5c and crluc6c) grown at low and high temperature as described for Figure 5B. The chimeric construct (C3_pro-5′-UTR)_{del c}:Ble-cRluc:C3-3′-UTR, which has a shortened c3 promoter and a replaced E-box (A), was transformed into wild-type cells, resulting in transgenic lines crluc5c and crluc6c. C, Based on the shortened c3 promoter region, the DREB1A-box at position −130 was replaced by six nucleotides representing a HindIII restriction site (pDI27; see “Materials and Methods”). D, Measurements of cRLUC activities in cells (crluc5d and crluc6d) grown at low and high temperature as described for Figure 5B. The chimeric construct (C3_pro-5′-UTR)_{del d}:Ble-cRluc:C3-3′-UTR, which has a shortened c3 promoter and a replaced DREB1A-box at −130 (C), was transformed into wild-type cells, resulting in transgenic lines crluc5d and crluc6d. E, Based on the shortened c3 promoter region, the DREB1A-box at position +72 was replaced by six nucleotides representing a HindIII restriction site (pDI28; see “Materials and Methods”). F, Measurements of cRLUC activities in cells (crluc5e and crluc6e) grown at low and high temperature as described for Figure 5B. The chimeric construct (C3_pro-5′-UTR)_{del e}:Ble-cRluc:C3-3′-UTR, which has a shortened c3 promoter and a replaced DREB1A-box at +72 (E), was transformed into wild-type cells, resulting in transgenic lines crluc5e and crluc6e.