Figure 1.

Spatial and temporal expression and protein structure of LlLIM1. A, LlLIM1 expression in different organs and different developmental stages of pollen were determined by semiquantitative RT-PCR. Three micrograms of lily total RNA extracted from root, leaves, stem, pistil, pollen grains, pollen tubes cultured in medium for 12 and 24 h, and anthers collected from flower buds of different lengths (10–20 mm, premeiosis; 20–30 mm, meiosis; 30–45 mm, microspore stage 1; 45–60 mm, microspore stage 2; 60–70 mm, mitosis; 70–90 mm, pollen maturation stage 1; 90–130 mm, pollen maturation stage 2; 130–150 mm, pollen maturation stage 3) were used for reverse transcription to obtain corresponding cDNAs for PCR, with the use of a LlLIM1 coding-region-specific primer set. rRNA amplified by the specific primer set was used as a loading control. B, Alignment analysis of the deduced amino acid sequences of LlLIM1, HaWLIM1 (sunflower; 85.1% similarity and 78.7% identity), AtWLIM1 (Arabidopsis; 84.7% similarity and 79.5% identity), NtWLIM1 (tobacco; 81.3% similarity and 76.2% identity), and OsWLIM1 (rice; 83.1% similarity and 77.9% identity). Black shading, identical and conserved amino acids; gray shading, similar amino acids; dark gray underline, LIM domain; asterisks, identical Cys or His residues for zinc finger motifs.