Figure 5.

Expression patterns of drought-responsive genes on LM mesophyll and vascular cells. RNA samples were extracted from laser microdissected cells and normalized against 18S rRNA (see “Materials and Methods”). Normalized templates were used for semiquantitative RT-PCR. The number of PCR cycles is indicated at right. PCR products were loaded on 4% agarose gels and visualized with ethidium bromide staining. This experiment was repeated twice using independent plant materials, and similar results were obtained.