Figure 7.

Quantitative real-time RT-PCR expression analysis of 30 primary metabolic and 12 alkaloid biosynthetic genes in flower buds of six opium poppy varieties. Plant variety abbreviations are the same as in Figure 1. Each bar represents the average of six measurements (two technical replicates on each of three independent biological samples) ± sd. Letters above bars indicate pair-wise differences identified by Tukey-Kramer multiple comparison tests. Bars lacking letters were not significantly different. BBE, Berberine bridge enzyme; CYP80B3, N-methylcoclaurine 3′-hydroxylase; DAHP, deoxy-d-arabino-heptosonate-7-phosphate; EPSP, 5-enolpyruvylshikimate-3-phosphate; NCS1, norcoclaurine synthase; 7OMT, reticuline 7-O-methyltransferase; SAM, S-adenosyl-Met; TNMT, tetrahydroprotoberberine-cis-N-methyltransferase.