Figure 3.

SIRT1 LKO mice are protected from physiological decline when fed a high-fat diet. (A–G) Body weight gain over time, WAT weight, liver weight, blood glucose, and insulin levels (both fed and fasted) and glucose tolerance were compared between wild-type and LKO mice on a high-fat diet. (H) Expression of ABCA1, FAS, and SREBP1c were compared between wild-type and LKO mice fed a high-fat diet or a CR diet by QRT–PCR. (I) Expression of SREBP1c was compared between wild-type and LKO mice fed a high-fed diet by Western blotting. Statistics was calculated by Student’s t-test. (*) P < 0.05; (**) P < 0.01.