Figure 7.

MSY-3 and Pbx binding to the myogHCE region in vivo. (A) Expression of three isoforms of Pbx in muscle at different times of maturation are evaluated by qRT–PCR. (B) ChIP analysis with control IgG, MSY-3, MyoD, and Pbx antibodies on chromatin from tibialis anterior muscle of young (2dd, 1w, and 2ww); adult (2MM); or denervated (2MMD) mice. Following immunoprecipitation, DNA enrichment was measured by qRT–PCR for myogenin promoter (top left), control IgH enhancer (top right), or −35-kb MyoD enhancer (bottom). (C) A sequential ChIP (RE-ChIP) experiment. Chromatin immunoprecipitated with MSY-3 was reincubated with anti Pbx (Pbx/MSY-3) or vice-versa (MSY-3 then Pbx). Results shown are representative of three independent experiments.