Figure 6.
Post-translational protein modification of the NuA4 core acetylation machinery choreographs DSB repair. (A) MG-132 alters the kinetics of histone H4 acetylation near an HO-induced DNA DSB. Chromatin immunoprecipitation (ChIP) assays with anti-H4-K8Ac were used to assess kinetics of histone H4 acetylation. Enrichment of histone H4-K8Ac was quantified by RT–PCR and normalized to the GEA2 internal control and also the local abundance of histone H3 (Supplemental Fig. S11). (B) NuA4 core acetylation machinery components show distinctive kinetics of recruitment to an HO-induced DNA DSB. The enrichment of tagged Esa1p, Yng2p, and Eaf1p after HO induction was assessed by ChIP assays with anti-HA. GAL-HO pdr5Δ strains were used in MG-132 experiments. (C) Proteasome and Rpd3C are recruited to an HO-induced DSB. Enrichment of tagged Rpn11p and Rpd3p after HO induction was assessed by ChIP with anti-HA.