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. 2008 Aug;17(8):1346–1353. doi: 10.1110/ps.034785.108

Figure 3.

Figure 3.

Ten percent SDS-PAGE displaying the effect of digestion by chymotrypsin and trypsin, separately and in combination, on bovine GDH. Lane 1 shows undigested GDH (55,561), lane 2 is GDH digested with chymotrypsin (200 μg/mL), lane 3 is GDH digested with chymotrypsin followed by trypsin (50 μg/mL), lane 4 shows GDH after trypsin digestion only, lane 5 is the result of trypsin digestion, followed by chymotrypsin. No attempt was made to remove BSA, the uppermost band (66,409) in all five tracks. The proteolytic enzymes were allowed to run off the gel.