Figure 1.

Absorption spectrum of coenzyme released from G6PD after TCA precipitation. Fifty percent (w/v) TCA was added to 3 mg/mL G6PD to final concentration of 15% (w/v). After being kept on ice for 1 h, the sample was centrifuged, and the supernatant containing released NADP⁺ was scanned between 200 and 600 nm.