Figure 4.

Stability properties of stripped G6PD. (A) Residual activity of G6PD without structural NADP⁺ incubated at 50 μg/mL in Tris-HCl (pH 7.6) with 5 mM EDTA at 4°C, 25°C, and 37°C. (B) Residual activity of an identical sample of G6PD incubated at 37°C after addition of 10 μM NADP⁺ to the buffer. In both cases, samples were withdrawn at intervals for assay under standard WHO conditions.