Table 1.
Primer sequences and annealing temperatures
|
Amplified segment |
Primer |
Annealing temperature |
| SPG31 Ex 1 | F1: ACCAGCTCACCGCCCAATC R1: GTATTAATAGCCCGAGCCACTGG |
56°C/30 cycles |
| SPG31 Ex 2 | F2: AAGATAGAGGTGCCCAAGGATG R2: TGAGAACTGTGATGAAGGAACTCC |
58°C/28 cycles |
| SPG31 Ex 3 | F3: GGAAGGATAGGGAGAAGGCTAC R3: CCTGAGGTGAGGTTTGAGGC |
58°C/28 cycles |
| SPG31 Ex 4 | F4: TTCAAAAGGGCAGTGTTGCTG R4: ATGACTTGCTGGAGGCAAATGA |
58°C/28 cycles |
| SPG31 Ex 5 | F5: GCCAAGCCCAATGCTCAGAG R5: GGTCCTTAGCCTGTTCTGTGTGG |
60°C/28 cycles |
| SPG31 Ex 6 | F6: AGCTCCCTCTTGGCCTTTGTC R6: GGACTGGGCCTCTCTCAATGA |
58°C/28 cycles |
| SPG31 Ex 7 | F7: GAGATTCAGGGAGACCCCAGC R7: GGAGAGAGAAAAGGCCATGTTTG |
60°C/28 cycles |
(F: forward primer; R: reverse primer)