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WAVE conditions
| Amplified segment | WAVE: oven temperature | time shift |
| SPG31 exon 1 | 65°C/70°C | no |
| SPG31 exon 2 | 54,5°C/57°C/58°C | no |
| SPG31 exon 3 | 59°C/63°C | 63°C: 2,0 |
| SPG31 exon 4 | 56°C/57°C/59°C | 59°C: 1,0 |
| SPG31 exon 5 | 58°C/61,5°C/64°C | no |
| SPG31 exon 6 | 61°C/62°C/63°C | no |
| SPG31 exon 7 | 58,6°C/60,6°C | no |
