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Time-course experiment of PS externalization due to CPT exposure. WT, LmTopIB-overexpressing and rescued (ΔtopS/ΔtopS)+topS strains were exposed to 5 μM CPT treatment for 24, 36 and 48 h. PS externalization was monitored by FACS. Dead cells were excluded by PI incorporation. Dot plots are representative of three independent assays.
