Table 3.
Housekeeping genes ranked in order of their expression stability | |||||||
Unoperated | M | 24 hr ligated | M | 2 wk ligated | M | 2 wk ligated + 3 day de-ligated | M |
ARBP | 0.434 | SDHA | 0.496 | SDHA | 0.792 | SDHA | 0.387 |
SDHA | 0.364 | GAPDH | 0.417 | YWHAZ | 0.641 | YWHAZ | 0.318 |
YWHAZ | 0.328 | ARBP | 0.352 | UBC | 0.562 | ARBP | 0.272 |
GAPDH | 0.243 | UBC | 0.278 | ARBP | 0.505 | HPRT | 0.207 |
HPRT | 0.196 | HPRT | 0.213 | GAPDH | 0.359 | UBC | 0.179 |
ACTB-UBC | 0.155 | ACTB-YWHAZ | 0.136 | ACTB-HPRT | 0.226 | ACTB-GAPDH | 0.124 |
Increasing stability from top to bottom; the two most stable control genes in tissue state, for example ACTB and UBC in unoperated tissue, cannot be ranked in order because of the required use of gene ratios for gene stability measurements. M is defined as the control gene-stability measure.