Table 1.

(a) Oligonucleotide primers for real-time quantitative reverse transcription–polymerase chain reaction (RT–PCR); (b) oligonucleotide primers for RT–PCR.

cDNA	Primers	Annealing (°C)	Cycles	Product (bp)
(a)
RIG-I	Forward: 5′-GTGCAAAGCCTTGGCATGT-3′	55	40	115
	Reverse: 5′-TGGCTTGGGATGTGGTCTACTC-3′
GAPDH	Forward: 5′-GCACCGTCAAGGCTGAGAAC-3′	55	40	142
	Reverse: 5′-ATGGTGGTGAAGACGCCAGT-3′
(b)
RIG-I	Forward: 5′-GCA TATTGACTGGACGTGGCA-3′	60	30	644
	Reverse: 5′-CAGTCATGGCTGCAGTTCTGTC-3′
FSP-1	Forward: 5′-AGCTTCTTGGGGAAAAGGAC-3′	60	30	200
	Reverse: 5′-CCCCAACCACATCAGAGG-3′
IP-10	Forward: 5′-ACCTCCAGTCTCAGCACCATG-3′	60	30	761
	Reverse: 5′- TGGGAGGATGGCAGTGGAAG-3′
MIG	Forward: 5′-TTCCTCTTGGGCATCATCTTGCTG-3′	58	30	336
	Reverse: 5′-GGTCTTTCAAGGATTGTAGGTGGA-3′
GAPDH	Forward: 5′-CCACCCATGGCAAATTCCATGGCA-3′	60	30	598
	Reverse: 5′-TCTAGACGGCAGGTCAGGTCCACC-3′

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IP-10, interferon-γ-inducible protein-10; FSP-1, fibroblast-specific protein-1; MIG, monokine induced by interferon-γ (MIG); RIG-I, retinoic acid-inducible gene-I; bp, base pairs.