Fig. 6.

High level of inflammatory cytokines and chemokines in lungs of neonatally primed mice following secondary challenge with respiratory syncytial virus (RSV) during adult life. Mice primed as adults or neonates were rechallenged with RSV during adult life 7 weeks post-priming. Mice previously naive were challenged once with RSV. Lungs were harvested 4 days after secondary challenge (or primary challenge for the naive group) and concentration of cytokines and chemokines in supernatants were determined by cytometric bead array assay or enzyme-linked immunosorbent assay and expressed as pg/ml of supernatant. Differences between adult- and neonatally primed groups are shown; *P < 0·05, **P < 0·01 and ***P < 0·001 by Student's t-test. Data are expressed for individual mice. The data shown are representative of two independent experiments.