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. 2008 Aug;124(4):480–488. doi: 10.1111/j.1365-2567.2007.02799.x

Figure 5.

Figure 5

Poly(I:C) induced interferon regulatory factor 3 (IRF3) activation in astrocytes. (a) Cells were incubated with 10 μg/ml poly(I:C). After stimulation, cell lysates were separated by native polyacrylamide gel electrophoresis (PAGE). Both monomeric and dimeric IRF3 were detected by rabbit anti-IRF3 antibody. (b) Cells were stimulated with 10 μg/ml poly(I:C) for the indicated time, fixed, permeabilized and stained with rabbit anti-IRF3 polyclonal antibody for 45 min at room temperature. Then cells were washed three times and incubated with 2·5 μg/ml Alexa Fluor-conjugated secondary antibodies (Alexa-594) for 1 hr at room temperature. Cells were analysed by confocal microscopy at × 40 magnification.