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Depletion of TAK activity from nuclear extracts with PITALRE antibodies. A HeLa cell nuclear extract was incubated as described in Materials and Methods with either antiserum against CDK7 or PITALRE or with GST-Tat-2 (wild type) or GST-C59A (Tat-2 mutant). Kinase assays for CDK7, PITALRE, or TAK (GST-Tat-2 and GST- C59A) remaining in extracts were then performed and analyzed on a 9% SDS/polyacrylamide gel.
