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. 2008 Aug 4;14:1414–1428.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reverse transcriptase polymerase chain reaction amplification of EMT-associated transcription response elements mRNAs during ARPE-19 and chick embryo retinal pigment epithelium cell differentiation RNA was isolated from undifferentiated and differentiated ARPE-19 or chick embryo retinal pigment epithelium (cRPE) cells and subjected to RT–PCR analysis to detect transcription response elements (TRE) mRNAs as described in Methods. Lanes 1 and 16 represent DNA standard ladders at 300, 400, and 500 bp, lanes 8 and 15 represent the positive controls for GADPH, and the remaining lanes represent primers specific for the following TFs: 2 and 9, Slug; 3 and 10, Snail; 4 and 11, Twist; 5 and 12, SIP-1; 6 and 13, SMAD-2; 7 and 14, LEF-1.