Table 2. Verification of the data by qRT-PCR.
| Gene | Primer sequence | Microarray fold change | qRT-PCR fold change |
|---|---|---|---|
| NDST4 |
5’-CCCCAAAGCCAAGATCATCA-3’ |
-3.7 |
-3.3 |
| |
5’-ACCTCAGAGCAGCTGGATCTTC-3 |
||
| CRH |
5’-GCAGCAGCAACACAATGTTATTC-3’ |
-2.3 |
-4.3 |
| |
5’-ACGTTTTCTCACAGGTCTACATTCTC-3’ |
||
| KCNC3 |
5’-CTTGTCACCGCCTGAGACCT-3’ |
-2.0 |
-2.7 |
| |
5’-GAGATTTGAAGCCCAGTGTCTT-3’ |
||
| STAT4 |
5’-GGTTGTCTGCTCTGCCATTC-3’ |
-1.9 |
-2.4 |
| |
5’-TTTGGGAATGTCAGGATATAGG-3’ |
||
| WNT5A |
5’-TTGCAGCGTATCACTGTTATGA-3’ |
-1.8 |
-2.5 |
| |
5’-TTCAAGTACACTGGGAACAGTTTT-3’ |
||
| GABRA1 |
5’-AAGAGGTCAAGCCCGAAACA-3’ |
-1.7 |
-2.5 |
| |
5’-AAATAGCAGCGGGAAGGCTAT-3’ |
||
| NQO2 |
5’-CCACGAAGCCTACAAGCAAAG-3’ |
-1.7 |
-3.1 |
| |
5’-AGTACAGCGGGAACTGAAATATCAC-3’ |
||
| CAMK2A |
5’-ATCACTGGCCTCTGTCCTTG-3’ |
1.8 |
2.3 |
| |
5’-ATGGGATCACTGGGCCTTACT-3’ |
||
| CREB5 |
5’-GCTCACCACTCACAGAACAGAC-3’ |
1.9 |
2.6 |
| |
5’-TTAAAAGAAAGGACGCATGGTA-3’ |
||
| DPYSL3 |
5’-GCTGACACCTGAGCCTGGAT-3’ |
2.0 |
2.5 |
| |
5’-GGAGAAAGCCTGGGAAGCTT-3’ |
||
| PIK3R1 |
5’-TCATTGAACAGCAAAGTAGGATTCA-3’ |
2.4 |
3.2 |
| |
5’-CGGTGGGCAAGCTACACTGTA-3’ |
||
| SCN3B |
5’-GACCATAGCTGCTTCCTTTTCT-3’ |
2.4 |
3.7 |
| |
5’-AGAAGCAGTGAGTGGGATTAGG-3’ |
||
| HRK |
5’-GGAGCCCAGAGCTTGAAAGG-3’ |
4.1 |
4.4 |
| |
5’-CCCCAGTCCCATTCTGTGTT-3’ |
||
| ALDH1A2 |
5’-AGTGTCTTCTGCAATGCAAGC-3’ |
5.4 |
6.3 |
| |
5’-CATTTCTCTCCCATTTCCAGAC-3’ |
||
| CRH |
5’-CTCACAGCAACAGGAAACTC-3’ |
-2.3 |
primers for in situ |
| |
5'-CTCTTACACAACCAAACTGACCAA-3' |
||
| GABRA1 |
5’-TATTGCCGTGTGCTATGCCTTTGT-3’ |
-1.7 |
primers for in situ |
| 5’-AGATGGGAATTACTGCGTTGAGAA-3’ |
The PCR primers were designed based on human gene sequences derived from GenBank. Primers for in situ hybridization probes were generated by PCR reactions using monkey corticotrophin-releasing hormone (CRH; accession number NM_000756) and gamma-aminobutyric acid A receptor, alpha 1 (GABRA1 from accession number NM_000806).