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. 2008 Aug 13;3(8):e2956. doi: 10.1371/journal.pone.0002956

Figure 4. G2/M response of caspase inhibited cells.

Figure 4

(a) G2/M content of C297A caspase catalytic inactivation mutant or wild-type BY4741 cells pre-treated for 30 minutes with 20 μM pan-caspase inhibitor z-VAD-FMK in response to 15 μg/mL nocodazole. (b) BY4741 cells pre-treated for 30 minutes with 20 μM Caspase 6 (z-VEID-FMK) and caspase 8 (z-IETD-FMK) specific peptide inhibitors before exposure to 15 μg/mL nocodazole. (c) Sub G1 population of BY4741 and Δyca1 cells either untreated or exposed to 15 μg/mL nocodazole over time (N = 3, ±S.E.M., P≤0.05 heteroscedastic unpaired 2-tailed t-test between paired *, ¶ and † symbols; note Figure 4a is a composite of multiple experiments).