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. 2008 Aug 13;3(8):e2956. doi: 10.1371/journal.pone.0002956

Figure 6. Downstream effects and oxidative stress.

Figure 6

(a) Bivariate flow cytometry analysis of H2O2 treated BY4741, BY4741 and Δyca1 cells dual stained with propidium iodide (PI) and ROS stain dihydrorhodamine 123 (DHR123). (N = 3, ±SEM). (b) 20s proteasome activity from BY4741 and Δyca1 lysates (N = 3, ±SEM). (c) Western blot for 3 biological replicates of BY4741, Δyca and Δrad23 (100 μg protein for each). Δrad23 appears as a negative control. The Rad23p antibody was obtained from Santa Cruz Biotechnology (SCBT, Santa Cruz, CA), β-tubulin (Iowa State University Hybridoma Bank) was used as a loading control.