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. 2008 Aug 13;3(8):e2969. doi: 10.1371/journal.pone.0002969

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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After alkaline methanolysis of MBM the upper biodiesel phase was separated and analyzed by GC-MS. GC-MS results show the alkaline methanolysis reaction produced various fatty acid methyl esters (FAME) which are typical components of biodiesel (see Table 1 for FAME composition). The two most abundant fatty acid methyl esters were analyzed quantitatively by using internal deuterated standards and calibration curve (Table 2).