Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Aug 13;3(8):e2969. doi: 10.1371/journal.pone.0002969

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

We performed alkaline methanolysis on scrapie brain spiked MBM as described under Methods and analyzed the reaction products as well as the inoculation samples by Western blot using monoclonal (mAb) 3F4. Results show no PrP detected in any of the 3 phases produced by the reaction (crude), nor in the inoculum we subsequently prepared by diluting each phase to 25 µg of total brain equivalent protein into 50 µl of 320 mM sucrose. After proteinase K digestion PrP^sc was clearly detected in MBM spiked with infected brain but not subjected to the reaction (MBM sc, crude +PK). 1% NBH served as a control, displaying a slightly higher molecular weight, since it was not proteinase K digested.