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. 2008 Aug 13;3(8):e2969. doi: 10.1371/journal.pone.0002969

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Control animals inoculated with 50 µl 1% scrapie BH exhibited clinical symptoms 75 days after inoculation and were sacrificed (1% sc brain). Animals inoculated with MBM sc began to display clinical symptoms around 75 days post inoculation and were sacrificed when moribund. All animals inoculated with Solid MBM Residue sc developed disease (166+/−13 days), even though no PrP^sc was detected by Western Blot in initial inoculum. Survival time of animals from all 3 groups was significantly shorter as compared to control animals (control: MBM c and Solid MBM Residue c; p<0.05; ANOVA on ranks). Animals inoculated with Biodiesel sc and Glycerol sc displayed no clinical symptoms within the time frame of the experiment (>200 days). Survival was comparable to control group animals (Biodiesel c, Glycerol c). Days of survival are calculated as mean values. (B) The range of time-to death values of hamsters inoculated with MBM sc and Solid MBM Residue sc is summarized showing the median, upper and lower quartiles and extreme values (outliers) for comparison of both groups.