Table 3. Solid MBM Residue remains infectious after alkaline methanolysis.
Inoculum | spiked with Infected Brain (Sacrificed Animals/Total animals) | Day Sacrificed |
Biodiesel phase | 4/14 ** | >200 |
Glycerol phase | 5/14 ** | >200 |
Solid MBM Residue+5% brain | 14/14 *** | 166+/−13 * |
MBM+5% brain | 8/8 *** | 72+/−8 * |
Brain Homogenate 1% | 6/6 *** | 75 |
mean+/−standard error
unsacrificed animals survived longer than 200 days, until study was terminated, without clinical scrapie symptoms
sacrificed animals exhibited clinical scrapie symptoms