Skip to main content
. 2008 Aug 15;4(8):e1000159. doi: 10.1371/journal.pgen.1000159

Figure 6. Loss of 1A-2 and 1A2′ reduces yar RNA accumulation during embryogeneis.

Figure 6

A. Ethidium bromide stained PCR products obtained from semi-quantitative PCR to evaluate ac, y, yar and Ras 64B RNA levels in wild type (Canton S), yΔ1A-2 (Δ1A-2) and yΔ1A-2/Δ1A-2′ (Δ1A-2/Δ1A-2′). Ras64B is constitutively expressed and serves as a control. The minus (−) RT lanes control for genomic DNA contamination. Different stages of embryonic and mixed pupal RNA were analyzed. B. Quantitative real time PCR (Q-PCR) was used to determine levels of yar mRNA accumulation from RNAs isolated during development from wild type and mutant lines. Individual transcript levels defined by Q-PCR were normalized to Ras64B for amount of input cDNA (ΔCT). A larger ΔCT indicates a reduction in RNA. Error bars indicate standard deviation of values obtained from analyses of three independently isolated RNAs. Significant changes in RNA accumulation relative to wild type are as indicated (*, P = <0.01; †, P<0.001, Student's two-tailed t-test).