Fig. 5.

βc and IL-5Rα partition to both the lipid raft and soluble, nonraft fractions. Cytokine-starved TF1 cells (2 × 10⁷) were either left unstimulated or stimulated with 10 ng/ml IL-5 for 30 min. Whole cell lysates (WCLs) were prepared with cold lysis buffer containing 0.1% Triton X-100 detergent on ice. Lysates were then processed for sucrose gradient fractionation as described in Materials and Methods. Twenty microliters of each fraction were loaded on an LDS-PAGE gel and analyzed by IB using antibodies listed on the right side of the panels. The lipid raft fractions are #1-3 and the soluble, nonraft fractions are #4-5.