Fig. 8.

Specificity of endocytosis inhibitors. Cytokine-starved TF1 cells (100,000 cells per tube) were serum-starved for 60 min and either left untreated (upper panels), treated with 0.45 M sucrose (middle panels), or 50 μg/ml NYS (lower panels) for 30 min to block each endocytic pathway. Tfr-488 and CTB-488 (both at 10 μg/ml) uptake was performed as described in the Materials and Methods. After acid washing the cells, we visualized the internalized transferrin and CTB by deconvolution microscopy.