Table 1.
Co-localization of IL-5 Receptors with Tfr-488, CTB-488, and LAMP1-488
| Labeled markers | # of cells examined | # of cells with + co-local | % positivea |
|---|---|---|---|
| TF1 cells | |||
| Tfr/Cy3-IL5 | 88 | 31 | 35 |
| Tfr/IL-5Rα | 24 | 16 | 67 |
| Tfr/βc | 27 | 21 | 78 |
| CTB/Cy3-IL5 | 268 | 121 | 45 |
| CTB/IL-5Rα | 41 | 7 | 17 |
| CTB/βc | 34 | 7 | 20 |
| LAMP/Cy3-IL5 | 157 | 28 | 18 |
| Eosinophils | |||
| Tfr/Cy3-IL5 | 61 | 29 | 47 |
| CTB/Cy3-IL5 | 53 | 16 | 30 |
| LAMP/Cy3-IL5 | 60 | 6 | 10 |
a
Percentage of cells with positive co-localization was calculated by counting number of cells with yellow vesicles (more than 2 vesicles per cell) over number of cells showing intracellular staining of Cy3-IL-5, βc, or IL-5Rα X 100. Cy3-IL-5 was used at 250 ng/ml.