Fig. 6.

Vγ2 cytotoxicity against Daudi B cell targets. Vγ2 effectors were generated by expanding PBMC (three HIV+ donors; one control) with IPP and IL-2 for 14 days and resting the cultures for 7 days prior to the cytotoxicity setup. (A) Vγ2 versus CD3 stains of the lymphocyte gates are shown for each of the four donors on the day of the toxicity experiment. (B) Vγ2 effectors are titrated over Daudi cell targets labeled intracellularly with 2 μM calcein-AM. Cytotoxicity was determined by assessing the presence of released fluorescent calcein in reaction supernatants.