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Survival of mutants affected in putative persister genes. Cells were grown to stationary phase in MOPS minimal medium in 96-well plates (final concentration, ∼1× 109 CFU/ml) and treated with 5 μg/ml ofloxacin for 6 h. Samples were diluted and spot plated on LB agar. The experiment was performed in triplicate, and error bars represent standard deviations. WT, wild type.
