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Survival of mutants with low levels of persistence exposed to different classes of antibiotics. One-milliliter cultures were grown to exponential phase in MOPS minimal medium and challenged for 3 h with 1 μg/ml ciprofloxacin (A), 10 μg/ml streptomycin (B), 100 μg/ml ampicillin (C), and 25 μg/ml benzalkonium chloride (D). Samples were diluted and spot plated on LB agar. The experiment was performed in triplicate, and error bars represent standard deviations. WT, wild type.
