FIG. 7.

Effects of ygfA and yigB overexpression on persister cell formation. E. coli BW25113 cells transformed with the pALS21 control (empty vector), pALS22 (yigB), and pALS23 (ygfA) were grown in MOPS minimal medium in the presence of 0.1 mM IPTG to stationary phase. The cultures were treated with ofloxacin for the indicated times. Samples were diluted and spot plated on LB agar. Closed squares, wild type; open squares, KLE921(pALS21); open diamonds, KLE922 (pALS22); closed diamonds, KLE923 (pALS23). The experiment was performed in triplicate, and error bars represent standard deviations.