FIG. 2.
PNU-286607 (QPT-1) activity in vitro against DNA gyrase (A) and TopoIV (B). Purified E. coli DNA gyrase (A) or TopoIV (B) was added to relaxed plasmid DNA (indicated by open circles) (A) or concatameric DNA substrate (indicated by multiple circles at the top of the gel) (B) in the presence of increasing amounts of PNU-286607 (QPT-1), and the relative activity of each enzyme was determined by gel electrophoresis and visualization by staining with EtBr. The IC50s of PNU-286607 (QPT-1) against gyrase and TopoIV are 9 μM and 30 μM, respectively.