TABLE 2.

Primers used for PCR amplification and sequencing

Target gene	Sequence	Amplicom size (bp)
pbp1a (SAG0298, gbs0288)	f1 5′-CGGAATTCATGGGATTTATTATCTTAGCTA-3′	2,209
	r1 5′-ACGTCGACTTAATTACCGTTAGGTACTGTA-3′
	f1b 5′-ACACCAAAGAAGAAATTCTTAC-3′
	f2 5′-TAAAGCAAAAATCTACTTATCC-3′
Sequencing primers	f2b 5′-GTAGTGAGAAAATGGCAGCGGC-3′
	f3 5′-GCCTACATGATGACGGATATGC-3′
	f3b 5′-CAAAATTCTGGACAGTCAAGTC-3′
	r2 5′-TCCAATCTGCACTGTATCCGCC-3′
	r3 5′-TAGCTGCTTTAGTACCAGTACC-3′
	r4 5′-CAGCGGCTTCAAGTGCTCTGAC-3′
	r5 5′-TGACTTTACCATTAGTCGCATC-3′
	r6 5′-TTTTATCTTGATACATCTGCTG-3′
pbp1b (SAG0159, gbs0155)	f1 5′-CGGAATTCATGTTTAAAGGTAATAAGAAGT-3′	2,314
	r1 5′-ACGTCGACTTATCGTTTTCCACCCAAAGTA-3′
	f1b 5′-GGTTTGGAGAGAGTAGCGG-3′
	f2 5′-CTATTGTATATTCTCCTTATAC-3′
Sequencing primers	f2b 5′-GTATACTATTAAAACTACTATC-3′
	f3 5′-TGATGTAAAAAACTATATGGAG-3′
	f3b 5′-CCTGTCCGTGTCTTTTCGAAAG-3′
	r2 5′-GTGTAGAAAGCATCAACCAAAC-3′
	r3 5′-GAGCAACTGACGTATCAATACC-3′
	r4 5′-GATCAATAGCAATTCCGTAAGG-3′
	r5 5′-TTTTTAAATCATGCTCTGAAAC-3′
	r6 5′-GTAAACCTGCAAGGAAAGCTGC-3′
pbp2a (SAG2066, gbs2020)	f1 5′-CGGGATCCATGAAATTATTTGATAAGTTTA-3′	2,338
	r1 5′-ACGTCGACCTATCTAAAGTAGTCCTTTAGA-3′
	f1b 5′-TGCTCTAAAAACAACCACCACC-3′
Sequencing primers	f2 5′-ATCTTAATAACTCTTATTTTGG-3′
	f2b 5′-GGTATGAAAAATAGATTAGCAG-3′
	f3 5′-TCCTGCTGTTTATACTTTAGAC-3′
	f3b 5′-ACTCGAATTGAGACAGCTAATG-3′
	r2 5′-CTGTCAAATAATGGTGTTTATC-3′
	r3 5′-ATGAGCGCGATGCATTATACCG-3′
	r4 5′-GTTCTTTATCTATTGACCATCC-3′
	r5 5′-TATAGCCATTATTGACAATATC-3′
	r6 5′-TCAAATTAGCAGCACTGGTTCC-3′
pbp2b (SAG0765, gbs0785)	f1 5′-CGGAATTCATGTTGAATCGTAAAAAAAGGT-3′	2,062
	r1 5′-ACGTCGACTTATTGTCCTGTGAACTGTGAA-3′
	f1b 5′-TTCATCTCAGTCTATCAAAGAG-3′
Sequencing primers	f2 5′-CTATTTCTACAGAAAAGGCAGG-3′
	f2b 5′-AGAAAGTATCTTGAAACAATAC-3′
	f3 5′-CAACTCTAATGGAATCGTTCGG-3′
	f3b 5′-TGGACAAACAGTTTCTACCTAC-3′
	r2 5′-CTATCTTATTTAGTGTTTTAGG-3′
	r3 5′-GATAGCCTCGATCAGTTAAAGC-3′
	r4 5′-CATGATCATTTTTCAGACCAGC-3′
	r5 5′-CTCGGTCATTCAGTGAATAGCC-3′
	r6 5′-TAGCGCTCACTGGAACTGCAGC-3′
pbp2x (SAG0687, gbs0277)	f1 5′-CGGAATTCGTGACTTTTTTTAAAAAGCTAA-3′	2,275
	r1 5′-ACGTCGACTTAATCTCCTATTGTAATTTTG-3′
	f1b 5′-AACTATACGACAGCTACAGGTC-3′
Sequencing primers	f2 5′-GTAGTGGGAATGTTCTTTTAGG-3′
	f2b 5′-TCTAAGCATTTTAACTCTACTG-3′
	f3 5′-AAGAAGCAGCTAGTAAAACACG-3′
	f3b 5′-GAAAATCCAGGTCATGTAGCGG-3′
	r2 5′-GAACCAGATTACGACGTAATTC-3′
	r3 5′-CAGATTTTACTGCAACTGATTG-3′
	r4 5′-ATGAGCTCATAGCGATAGTTAC-3′
	r5 5′-TTGCAGAGGCTAGAGTCATTAC-3′
	r6 5′-CCGCCCTACGTTCTGTTGTTGC-3′
	r7 5′-AAGACAATCCTGAACCTGAACTTCC-3′
	r8 5′-TATCTGTACCAACGATGATGAC-3′