FIG. 2.
Plasmid profile and Southern hybridization analysis of blaIMP-15. (A) Plasmid profiles of the MβL-producing P. aeruginosa isolates. Plasmids were prepared from 10 isolates and were subjected to agarose gel electrophoresis. (B) Southern hybridization analysis. The 275-bp fragment amplified from blaIMP-15 by PCR was used as the DNA probe. Lanes: 1, plasmid R6K; 2, plasmid RP4; 3, plasmid R1; 4, pMG229; 5, pUD21; 6, strain 4658; 7, strain 4663; 8, 4667; 9, strain 4677; 10, strain 4679; 11, strain 4680; 12, strain 4682-1; 13, strain 4696; 14, strain 4698; 15, strain 4703. Ch, chromosomal DNA.