TABLE 1.
Sequences of primers used in this study
| Target | Primer name | Sequence (5′ to 3′) | Amplicon size (bp) | Annealing temp (°C) | Reference or source |
|---|---|---|---|---|---|
| blaIMP | IMP-F | GGAATAGAGTGGCTTAATTC | 275 | 58 | This study |
| IMP-R | GCCAAGCTTCTATATTTGCG | ||||
| blaVIM | VIM-F | GTGTTTGGTCGCATATCGC | 380 | 58 | This study |
| VIM-R | CGCAGCACCAGGATAGAAG | ||||
| intI1 | intl1a | CGTTCCATACAGAAGCTGG | 1 | ||
| 5′ CSb | GGCATCCAAGCAGCAAG | 60 | 17 | ||
| 3′ CSb | AAGCAGACTTGACCTGA | 17 | |||
| aadA1 | aadA1-F | ATGAGGGAAGCGGTGATCG | 792 | 60 | This study |
| aadA1-R | TTATTTGCGGACTACCTTG | ||||
| aacA4 | aacA4-F | ATGACTGAGCATGACCTTG | 508 | 56 | This study |
| aacA4-R | TGCGTGTTCGCTCGAATGCC | ||||
| aadA6 | aadA6-Fb | ATGAGTAACGCAGTACCCGC | 634 | 54 | This study |
| aadA6-Rb | CCCCAGTGGCAACGATATCC | ||||
| aacA7 | aacA7-F | ATGGATAGTTCGCCGCTCGT | 362 | 58 | This study |
| aacA7-R | GAGGCGAATTCGGTGCATCC | ||||
| qacH | qacH-F | CTGGCTCTTTCTGGCTATTG | 325 | 60 | This study |
| qacH-R | TCAATGTGCGCTGACCTTGG | ||||
| oxa2 | oxa2-F | ATGGCAATCCGAATCTTCGC | 828 | 60 | This study |
| oxa2-R | TTATCGCGCAGCGTCCGAGT | ||||
| orfD | orfD-Fb | CAGTATCTCAAACGCTGTG | 223 | 56 | This study |
| orfD-Rb | AATGTTAGAGCCAGAAGCC | ||||
| qacEΔ1 | L1a | GCCCTACACAAATTGGGAGA | 381 | 64 | 12 |
| R1a | AACACCGTCACCATGGCGCCG | 12 |
a
Primers used for PCR amplification and sequencing of intI1 (partial sequence) and qacEΔ1 genes of 5.4- and 1.4-kb class 1 integrons.
b
Primers used for PCR amplification and sequencing of the variable region of 1.4-kb class 1 integron.