TABLE 2.
PCR primers used in this work
| Primer | Sequence (5′-3′) | Target gene or region | Referencea |
|---|---|---|---|
| imp-BE1 | CAYGGTTTGGTGGTTCTTGTAA | blaIMP variants | |
| imp-BE2 | CCTTTAACVGCCTGYTCTYMT | ||
| hep58 | TCATGGCTTGTTATGACTGT | Cassette arrays in class 1 integrons | 19 |
| hep59 | GTAGGGCTTATTATGCACGC | ||
| catB3-R | CTGTACCGCCCAACTTTGAT | catB3 gene cassette | |
| K31-R | CAATCCCGCTAAGAAGAb | ||
| Tn3P-F | GGGTTCTGGTCGAAGCTGCT | Tn21-like tnp regions | |
| SP1 | ATCCTGGCGGATTCACTACC | In 5′-CS near IRi | |
| IS26-F2 | TGGCAAACTGAAACGGATAA | IS26 | |
| IS26-R2 | GCAGCCGTTTTTCCATTTCA | ||
| 3CS-F | CTATTGGTCTCGGTGTCG | 3′-CS | |
| 3CS-R | ATCGTTCAGGTAGCCCAC | ||
| CR1-R | TTCAGGGTGCTATTGCTC | ISCR1 | |
| CR1-F | ACAAATCGGAAGGTCTC | ||
| IS1326-R | GATTTGGTGATTCTGGATG | IS1326 | |
| IS6100-R | GTAGGCTGTCGCTCTG | IS6100 | |
| qB2reg-1 | CGGTCGGCTTTTTCGCAA | qnrB2 region | |
| qB2reg-2 | GCCCATCTGACTTGCTAT | ||
| orf98-F | GAGAGTTGGTAGAAGGTGGTTGA | orf98 | |
| mphR-F | TTGCGGTCCCATAGAGTTCAC | mph region | 16 |
a
All primers listed were designed as part of this work except where referenced.
b
The underlined C was mistakenly replaced by CC in the primer used.