FIG. 4.
Effect of the Cj1211 mutation on the development of FQr mutants in cultures treated with ciprofloxacin. C. jejuni NCTC 11168 and JB201 were grown in separate culture tubes without ciprofloxacin for 8 h, and ciprofloxacin was then added to the cultures to a final concentration of 1 μg ml−1. The initiation time of ciprofloxacin treatment is indicated by an arrow. Open and solid circles indicate the total bacterial counts of JB201 and NCTC 11168, respectively, while the open and solid bars represented the numbers of FQr mutants of NCTC 11168 and JB201, respectively. The results show the means ± standard deviations of data for triplicate samples in a single experiment. Similar patterns of results were obtained in three independent experiments.