TABLE 2.
Susceptibility of P. aeruginosa ATCC 15442 biofilms to single-agent treatments, as determined by high-throughput screening (using CBDs)a
| Metal ion or biocide (concn unit) | MBCb
|
||
|---|---|---|---|
| 5 min (in ddH2O) | 30 min (in ddH2O) | 24 h (in 10% TSB-ddH2O) | |
| Ag+ (mM) | >19 | >19 | >19 |
| Cu2+ (mM) | >32 | >32 | 32 (16 to 32) |
| Al3+ (mM) | >76 | >76 (38 to >76) | 76 (76 to >76) |
| SeO32− (mM) | >16 | >16 | >16 |
| Zn2+ (mM) | >31 | >31 | >31 |
| Polycide (ppm) | >3,200 (50 to >3,200) | 1,800 (100 to >3,200) | 400 (50 to 800) |
| Stabrom (ppm) | 250 (31 to >500) | 250 (16 to >500) | 94 (63 to >1,600) |
| Isopropyl alcohol (%) | 0.50 (0.25 to 0.50) | 0.50 (0.25 to 0.50) | 0.50 (0.25 to >0.50) |
| Virox (ppm) | 313 (156 to 5,000) | 313 (156 to 2,500) | 625 (313 to 2,500) |
a
Note that these assays were performed using a high-throughput screening method that judges killing qualitatively. Incomplete killing of microbial populations may still occur with short exposure times, but this is not detected by the high-throughput method employed here.