TABLE 2.
Primers used for PCR and sequencing
| Primer | Sequence (5′-3′)a | Primer binding site(s)b |
|---|---|---|
| yaiU_F | AGCGCCTACACCGATGCTAA | −2344 |
| yaiW_R | TGACTACTACAGCCAGCCAA | 1306 |
| PR_seq2 | TCAGGTACTGGAACTTCGTG | −1804 |
| PR_seq3 | GGTACGTCCATCCCGATGAT | −1251 |
| PR_seq4 | TACCGTTCCCTGATCGAGCA | −644 |
| PR_seq5 | GCGTCACAACGTAGGTCACA | −116 |
| PR_seq6 | GGTGATCGGTGTGCTGAACA | 447 |
| PR_seq7 | GCGCATTCTCTATTTGCAGG | 966 |
| sbmA_1 | CTCGGCATAACGATCAACGA | −466 |
| sbmA_2 | TGGTCGCTGGACTATCTGAT | 176 |
| EcoRIsbmA_F | GTGAATTCGTCACAGCAGCCGTAATAA | −45 |
| XbaIsbmA_R | GCTCTAGACGCCGACATAACGTTTCCTT | 1244 |
| LTsbmA_F | CGTCACAGCAGCCGTAATAAAGATAAGAAGTTA GCAGGAGAGCATGTGTAGGCTGGAGCTGCTTCG | −46 |
| LTsbmA_R | CGGGACGCCGCCGACATAACGTTTCCTTCTGTT AGCTAAAAGAGTGCATATGAATATCCTCCTTAG | 1252 |
| Arb1 | GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT | Arbitrary |
| Arb6 | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC | Arbitrary |
| Arb2 | GGCCACGCGTCGACTAGTAC | Arb1 and Arb6 |
| TL | TCCATTGCTGTTGACAAAGGGAAT | Mini Tn10 (Tetr) |
| TR | ACCTTTGGTCACCAACGCTTTTCC | Mini Tn10 (Tetr) |
| Universal | GACAAGATGTGTATCCACCTTAAC | Mini Tn10 (Tetr) |
| pBAD_F | TTAGCGGATCCTACCTGACG | pBAD30 |
| pBAD_F2 | CGCTTCAGCCATACTTTTCA | pBAD30 |
| pBAD_R | ACGGCGTTTCACTTCTGAGT | pBAD30 |
a
Restriction enzyme cleavage sites are underlined.
b
For the first 14 primers, the primer binding site is indicated as the position of the 5′ nucleotide in relation to the first nucleotide in the start codon of sbmA. A minus indicates nucleotides upstream of the start codon.