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. 2008 May 27;52(8):2771–2779. doi: 10.1128/AAC.01671-07

FIG. 1.

FIG. 1.

Effects of CBAs on HTLV-1 (TAX/REX) mRNA expression were evaluated 4 weeks after in vitro HTLV-1 infection of human PBMCs from healthy donors through exposure to HTLV-1-infected irrMT-2 cells. The lane numbers for panels A (PRM-A), B (UDA), and C (HHA) represent following experimental conditions. Lane 1, uninfected PBMCs; lane 2, untreated HTLV-1-infected human PBMCs; lane 3, PBMCs pretreated with 1.2 μM PRM-A before cocultivation with irrMT-2 cells; lane 4, irrMT-2 cells pretreated with 1.2 μM PRM-A before cocultivation with PBMCs; lane 5, PBMCs and MT-2 cells pretreated with 1.2 μM PRM-A before cocultivation; lane 6, PBMCs and MT-2 cells treated with 1.2 μM PRM-A at the initiation of the cocultures; lanes 7 to 10, conditions identical to lanes 3 to 6 but treated with 10-fold-higher CBA concentrations (12 μM PRM-A); lane 11, chronically HTLV-1-infected MT-2 cells. The control lanes in panels B and C are in the same order, and in panel B, lanes 3 to 6 and 7 to 11 represent 0.11 and 1.1 μM UDA, while in panel C, lanes 3 to 6 and 7 to 11 represent 0.02 and 0.2 μM HHA. The mRNA expression of the GAPDH housekeeping gene is shown for each assayed compound. Lane M, 1-kb (1.5 to 0.1 kb) molecular ruler (Bio-Rad). NT, no template.