TABLE 3.
Validation of a subset of predicted polymorphic regions by PCR
| Region (bp)a | CDS/gene | Annotated function | PCR resultb
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| ATCC 3502 | FRI-H1A2 | CDC51303 | CDC297 | CDC1882 | CDC1903 | CDC5328 | |||
| 363530-364052† | CBO0310/fhuD1 | Putative ferrichrome binding protein | + | - | + | + | + | + | + |
| 736511-737071* | CBO0640 | GntR-family transcriptional regulator | + | + | - | - | - | - | - |
| 748682-749599* | CBO0652 | Putative helicase | + | + | - | - | - | - | - |
| 984713-985577* | CBO0881/bglC | β-Glucoside-specific IIabc component | + | + | - | - | - | - | - |
| 1152354-1153119* | CBO1050 | Composite ABC transporter | + | + | - | - | - | - | - |
| 2300063-2300564† | CBO2156 | Putative membrane protein | + | - | + | + | + | + | + |
| 2449311-2449822† | CBO2293 | Short-chain dehydrogenase | + | - | + | + | + | + | + |
| 2601914-2602630* | CBO2468 | Methyl-accepting chemotaxis protein | + | + | - | - | - | - | - |
| 2763745-2764337* | CBO2615 | Rhodanese-like protein | + | + | - | - | - | - | - |
| 3121483-3121982† | CBO2932 | Putative exported protein | + | - | + | + | + | + | + |
| 3370351-3370902* | CBO3167 | Putative ABC transporter permease | + | + | - | - | - | - | - |
a
The nucleotide location based on the genome sequence of ATCC 3502. *, region predicted to be present in C. botulinum strain FRI-H1A2 and absent in C. botulinum ha−/orfX+ A1 strains by microarray analysis; †, region predicted to be absent in C. botulinum strain FRI-H1A2 and present in C. botulinum ha−/orfX+ A1 strains by microarray analysis.
b
The presence (+) or absence (-) of a band of appropriate size is indicated using PCR primers listed in Table S1 in the supplemental material under “putative polymorphic region validation.”