TABLE 2.
Analysis of proteins (from 2D gels) whose expression levels were significantly different under two different conditionsa
| Protein no. | Locus tag(s) | pI (theoretical) | Mass (kDa) (theoretical) | Identification |
|---|---|---|---|---|
| 1 | A3089 | 4.9 | 69.9 | DnaK (HSP70; chaperone) |
| 2 | A0706 | 5.0 | 57.3 | GroEL (HSP60; chaperone) |
| 3 + 4 + 5 | B2360 | 4.98 | 44.8 | Flagellin (FliC) |
| 6 | A2752/A2054 | 5.21/5.26 | 32.5/30.9 | UTP-glucose-1-phosphate uridyltransferase (GalU)/protein translation elongation factor TS |
| 7 | B1369 | 6.38 | 43.0 | β-Ketothiolase |
| 8 + 9 | A1381 | 5.96 | 20.0 | PhaP1; isoforms |
| 10 | A1574 | 7.29 | 27.5 | Ankyrin repeat protein |
| 11 | Not identified | |||
| 12 | PHG089 | 6.82 | 26.2 | NAD-reducing hydrogenase diaphorase moiety small subunit (HoxU) |
| 13 | A1618 | 6.05 | 16.6 | Conserved hypothetical protein |
| 14 | A0750 | 5.7 | 12.3 | Nitrogen-regulatory protein PII |
| 15 | A0750/A0320 | 5.87/5.7 | 12.2/12.3 | Nitrogen-regulatory protein PII/nitrogen-regulatory protein PII (GlnK) |
| 16 | A0705 | 5.8 | 10.5 | Cochaperonin GroES (HSP10) |
| 17 | A0320 | 5.87 | 12.2 | Nitrogen-regulatory protein PII (GlnK) |
| 18 | B0194 | 7.93 | 12.6 | Conserved hypothetical protein |
| 19 | A0386 | 7.30 | 13.6 | Sigma 54 modulation protein S30EA |
a
Identification of isolated proteins was done by tryptic digestion, MALDI-TOF, and, finally, comparison of the resulting mass spectrum data with hypothetical spectra out of the R. eutropha H16 data bank by in silico digestion.