TABLE 3.

Summarized results of the investigations of R. eutropha H16, ΔphaP1 ΔphaP1234, PHB⁻4, and HF09 strains

Treatment and R. eutropha strain/genotype	FliC detectiona	Flagellation levelb	PHB (% cell dry weight)	Concn
				C sourcec	N sourced
MM
64 h
H16	+	++	34	−	−
ΔphaP1	+	++	27	−	−
ΔphaP1234	+		10	−	−
PHB−4	−		0	+	−
HF09	±	±	36	−	−
72 h
H16	−	±	30	+	+ (puls)
ΔphaP1	+	++	22	+	+ (puls)
ΔphaP1234	+		6	+	+ (puls)
PHB−4	−		0	±	+ (puls)
HF09	−	±	34	+	+ (puls)
TSB
64 h
H16	−	±	0	±	+
ΔphaP1	−	±	2	±	+
ΔphaP1234	−		0	±	+
PHB−4	−		0	±	+
HF09	−	±	0	±	+
72 h
H16	−	−	1.5	±	++ (puls)
ΔphaP1	−	−	2	±	++ (puls)
ΔphaP1234	−		0	±	++ (puls)
PHB−4	−		0	±	++ (puls)
HF09	−	±	0	±	++ (puls)

^a+, flagellin detected; ±, eventually small amounts of flagellin detected; −, no flagellin detectable.

^b++, strongly flagellated; ±, sporadically flagellated; −, nonflagellated.

^c+, sufficient; ±, concentration hardly predictable; −, depleted.

^d++; very high; +, high; −, depleted. Puls means provision of NH₄Cl to a concentration of 0.1% (wt/vol).