FIG. 2.
Results of TaqMan real-time PCR analysis targeting the chromosome of B. anthracis. Serial tenfold dilutions of strain K1694 (Ames) spores were performed, and spores were exposed to 2.5 × 106 rads of gamma radiation. Untreated (A) and irradiated (B) spores at concentrations ranging from 107 to 101 CFU/ml (105 CFU [▪], 104 CFU [□], 103 CFU [•], 102 CFU [○], 10 CFU [▴], 1 CFU [*], and 0 CFU [x]) were detected by real-time PCR. The results were plotted as the average relative fluorescence of triplicate wells versus the cycle number.